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Chapter 2 Microscopy

2.3

Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color)

Attach the items required for the polarization microscopy under the episcopic

illumination to the microscope. (See Table 2.1.)

Focus on the sample with the bright-field microscopy under the episcopic illumination.

(See Pages 16 to 17.)

Set the microscope for the polarization microscopy under the episcopic illumination.

1 Turn the revolving nosepiece to place the

objective of a desired magnification into the optical

path. (See 3.9.2.)

2 Push in the polarizer/analyzer unit to the second

click-stop position and place it into the optical

path. (See 3.13.1.)

3 Turn the polarizer rotation ring to the crossed

Nicol’s position. (See 3.13.2.)

4 To perform the sensitive color polarization

microscopy, insert the λplate into the

polarizer/analyzer unit and place it into the optical

path. (See 3.14.1.)

5 Turn the brightness control dial to adjust the

brightness of the episcopic illumination. (See

3.2.1.)

6 Adjust the brightness with the desired ND filter in

the filter turret. (See 3.10.)

7 Turn the polarizer rotation ring to adjust the

polarization while observing the image.

Return to the bright-field microscopy under the episcopic illumination.

1 Pull the analyzer/polarizer unit to the first

click-stop position to remove the

analyzer/polarizer from the optical path. (See

3.13.1.)

2 Both the λplate and the polarizer/analyzer unit are

removed from the optical path. (See 3.14.1.)

3 Turn the brightness control dial to adjust the

brightness of the episcopic illumination. (See

3.2.1.)

4 Adjust the brightness with the desired ND filter in

the filter turret. (See 3.10.)

3-5

3-6

3-3, 7 3-2

3-4

3-1

1 2 ... 17 18 19 20 21 22 23 24 25 26 27 ... 106 107

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