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Chapter 3 Operation Details
32
Table 3.3-1 Selecting the microscopy method
Microscopy Revolving
nosepiece
Objective Items required for the optical
path
BD changeover
lever
Remarks
BF
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
⎯
BF (Press) Normal bright-field microscopy under the
episcopic illumination.
DF
microscopy
under the
epi
illumination
5 revo. only BD objective only
⎯
DF (Pull) Pull out BD field changeover lever to perform
dark-field microscopy under the episcopic
illumination.
Pull out BD field changeover lever to fully
auto-open field/aperture diaphragm (The dial
position remains the same).
Pull out BD field changeover lever to exclude
polarizer/analyzer unit or fluorescent unit when
it is in the optical path.
Simplified
polarization
microscopy
under the
epi
illumination
5 revo.,
6 revo.,
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• MA2-PA/MA2-UPA
polarizer/analyzer unit
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
BF (Press) Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
DIC
microscopy
under the
epi
illumination
5 revo.,
6 revo.
LU EPI objectives or LU
BD objective only
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• BD objective
unavailable for 6
revo.
For 5 revo.:
• MA2-PA polarizer/analyzer
unit
• L-DIHC/L-DIC slider (single
NR method)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
For 6 revo.:
• MA2-UPA polarizer/analyzer
unit
• LV-DIHC/LV-DIC slider
(Senarmont method)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
BF (Press) Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol’s
position. Place DIC prism into optical path to
perform DIC microscopy.
To adjust the contrast, turn the prism setting
dial for the single NR method, whereas turn the
polarizer rotation ring for Senarmont method.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
Epi-fl
microscopy
5 revo.,
6 revo.,
7 revo.
LU EPI objectives or LU
BD objective only
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• MA2-FL fluorescent unit BF (Press) Place a desired fluorescent unit G, B, BV or V
into optical path to perform epi-fl microscopy.
BF
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
⎯
BF (Press) Normal bright-field microscopy under the
diascopic illumination.
Simplified
polarization
microscopy
under the
dia
illumination
5 revo.,
6 revo.,
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• T-P2 polarizer
• MA2-PA/MA2-UPA
polarizer/analyzer unit (for 6
revo., D-DA analyzer is also
available)
• For the sensitive color
polarization microscopy,
TI-DIC λ plate available (for 6
revo., D-LP λ plate is also
available)
BF (Press) Set bright-field microscopy under diascopic
illumination. And then, attach polarizer to
diascopic pillar illuminator and insert analyzer
into MA200 main body to make crossed Nicol’s
position. (For polarizer/analyzer unit, the
analyzer part is used.)
Attach TI-DIC λ plate to supporting pillar for
dia-Illuminator 100W. For 6 revo., D-LP λ plate
is also available.
- ECLIPSE MA200 1
- Introduction 3
- Safety Precautions 4
- WARNING 5
- 8. Air vents 6
- CAUTION 7
- 8. Cable routing 8
- Contents 10
- Part Name and Function 12
- Microscopy 16
- Chapter 2 Microscopy 18
- ■ Sensitive color microscopy 23
- 2.5 Epi-fl Microscopy 24
- (See Table 2.1.) 26
- Adjust the focus again 28
- Operation Details 31
- 3.2 Illumination 32
- Chapter 3 Operation Details 33
- 3.4 Eyepiece Tube 35
- Eyelevel riser 36
- 3.6 Adjusting the Diopters 37
- ■ Sample holder 39
- 3.10 Filter 42
- 3.15.3 Interference color 49
- 3.16.3 Interference color 50
- Figure 3.20-2 Scale slider 56
- Figure 3.25-2 Objective info 68
- Name of the objective 68
- Nosepiece address 68
- NA value of the objective 68
- WD value of the objective 68
- Assembly 70
- Chapter 4 Assembly 71
- 4.1 About the System 72
- [Note 2] 75
- [Note 1] 75
- CAUTION for heat 79
- CAUTION 79
- 4.5 Revolving Nosepiece 80
- 4.6 Stage 82
- 4.7 Objectives 83
- 4.8 Eyepiece Tube 83
- 4.9 Eyepieces 83
- 4.11 Filters 84
- 4.13.1 MA2-λP λ plate 87
- 4.15 External Light Source 89
- WARNING 89
- Preparation for attachment 91
- ■ Attaching the DS-L2 97
- 4.17 Eyelevel Risers 98
- 4.18 Camera Adapter 99
- 4.19 Power Cord 99
- Troubleshooting 100
- Care and Maintenance 104
- 6.3 Storage 105
- Specifications 106
- Chapter 7 Specifications 107
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