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Chapter 3 Operation Details

3.19 Using the Fluorescent Unit (MA2-FL)

Attach the fluorescent unit to the MA200 main body to

perform the epi-fl microscopy.

A fluorescent unit consists of two types of optical

components: an excitation light filter (EX filter), a

barrier filter (BA filter). You can use the four types of

combination, "G", "B", "BV" or "V" as shown in the

table below. Select the fluorescent unit suitable for

your purpose and sample.

Figure 3.19-1 Fluorescent unit

To perform epi-fl microscopy using the

C-HGFI/C-HGFIE HG Precentered Fiber

Illuminator as the external light source, screw the

compensation filter supplied with the fiber

adapter into the filter attachment of the

fluorescent unit. (Refer to 4.15.1.)

Fluorescent unit Characteristics of EX filter (nm) Characteristics of BA filter (nm)

MA2-FL G 510-560 590

MA2-FL B 450-490 520

MA2-FL BV 400-440 470

MA2-FL V 380-420 450

3.19.1 Inserting/removing the fl filter from the optical path

Figure 3.19-2 Inserting/removing the fluorescent

unit from the optical path

Push in the unit attached on the microscope. When it

hits the second click-stop position, the fl filter enters

the optical path.

Pull out the unit. When it hits the first click-stop

position, the fl filter is removed from the optical path.

Under this condition, the compensation filter attached

to the fluorescent unit enters the optical path to

reduce glare in the bright-field observation. (Refer to

4.15.1.)

Fluorescent unit

Second click-stop position

First click-stop position

Excitation

ht filte

Barrier filter

Compensation filter

attachment

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